maxpar human t cell phenotyping panel kit standard biotools  (fluidigm)

Journal: Nano Today

Article Title: Immune profiling and tracking of two-dimensional transition metal dichalcogenides in cells and tissues

doi: 10.1016/j.nantod.2023.102084

Figure Lengend Snippet: Fig. 1. Workflow of the study. Schematic representation for MoS2 and WS2 detection in cells and tissues using mass cytometry-based detection of stable isotope masses from 75 to 209 Da. i) Study workflow showing basic biological characterization of TMDs on whole blood and peripheral blood mononuclear cells. The impact of TMDs was assessed ex vivo on 16 human immune cell types by single-cell mass cytometry and imaging mass cytometry. Graphical representation of all metal tags used for single-cell mass cytometry experiments assessing immune cell phenotyping markers (pink box), viability, palladium-based barcoding (orange), and DNA staining. MoS2 and WS2 were detected in the 95Mo and 182W channels, respectively. ii) TMD in vivo testing from biodistribution to in vivo cell interactions by single- cell mass cytometry and multiplexed ion beam imaging.

Article Snippet: The barcoded sample was stained with Cell-ID Cisplatin (Fluidigm, CA, USA) 1:1000, Maxpar Human Peripheral Blood Phenotyping Panel Kit (Fluidigm, CA, USA) following the manufacturer staining protocols.

Techniques: Mass Cytometry, Ex Vivo, Imaging, Staining, In Vivo